Ultrasonic of experiment research application is broken Wei Moyong of experiment of bacili of gemma of Su Yun gold 1 Su Zhixian 2 (1. Sichuan visits silk school, 637000 2 is filled south Sichuan. Xi Hua Normal University, fill south Sichuan 637000) the result makes clear: Below experiment condition, ultrasonic is broken what the broken rate that achieves 95% above needs time to be 30~ 50min, especially bacterium fluid chroma is every milliliter 5 billion, with 510 luffing lever broken, 40min broken rate can be amounted to 99.9% . And bacterial gemma, no matter be,use a lever *6 or 510, its are broken the effect is not apparent.
Bt) it is the research on agriculture most, a application is the widest kind microbial, as the development of biology technology, more and more thorough to the research of bacili of gemma of Su Yun gold. In a few research, need cell of effectively bacili of gemma of gold of broken Su Yun. Current, application has two kinds of physics and chemistry at the method with broken cell, in physics there basically is thick liquid of vitreous divide evenly in broken method implement cell of broken, high pressure broken, high speed organizes broken, liquid nitrogen triturator ultrasonic is broken, broken etc, this experiment applied commonly used ultrasonic broken law, respectively to Su Yun the nutrient cell of bacili of Jin Ya Bao and gemma had fragmentation test, with period help somewhat to research.
1 material and method 1.1 data income is little, can adopt the measure that distributes zephyr danger fund 2 times the manufacturing supply of pod of flexibility of 3.2 analysises production, reasonable adjusting control. No matter be rice takes price agitate, conduct propaganda agitate or administration to drive,want measurable, avoid to appear to be fooled and go up start pod great war, to finally internecine.
Silkworm industry production produces the inevitable trend that change is development of chrysalis silk industry to industrialization by traditional type of production, also be the mark that advances industrialization process and target. We are a breakthrough with system innovation only, rely on / company + farmer " silkworm course of study changes management pattern, enhance a farmer the main body position in the industry, make the farmer becomes those who produce the market principal part that manage and whole industry to be benefited truly main body, make broad silkworm raiser takes off deficient to become rich thereby go straight towards well-off, to build socialism new rural area lays a foundation.
Bacili of gemma of Su Yun gold saves silk school microbial lab to save for Sichuan.
7532, do bacterium to plant rejuvnation is used.
Ultrasonic crusher is produced by Inc. of science and technology of biology of Ning Bo new Zhi.
The boiler of sterilization of box of education of electric heat constant temperature, freezer, high pressure, smooth Min, bottle that overcome a family name, straw, tube.
Beef creams albumen peptone liquid is fostered base foster with solid base (nutrient agaragar) .
Will refrigerant bacterium is planted have an inoculation creams at beef albumen peptone liquid is fostered base education 18~20h, to 2, 3 age remove silkworm to add feed, wait for silkworm after sending ill death, have bacterial depart and identification, affirm for cause of disease of silkworm damping off abrupt of one bacili of gemma of Su Yun gold pours subspecies.
The experiment uses the preparation of fluid: Rejuvnation is planted in measuring have an inoculation to overcome family name bottle at nutrient agaragar certainly, constant temperature 37*C fosters 14h and 24h respectively, wash liver mosses issueing bacterium with right amount physiological saline, make up experiment place to require concentration, 4*C freezer is saved.
Of fluid of cell of 1 education 14h broken the test that takes education 14h chooses with bacterium fluid 200ml 56, the luffing lever of 510, applied job 5s rests the operation pattern of 5s, to different pH indicator bacterium fluid is actual and broken 10, 20, 30, 40, after 50min, have viable bacteria count.
Experiment of 24 hours chooses with the 200ml of bacterium fluid 56, the luffing lever of 510, apply working 5s to rest the operation pattern of 5s, to different pH indicator bacterium fluid is actual and broken 10, 20, 30, 40, 50, after 60min, have viable bacteria count.
Consult " microbial experiment manual " the method of intermediary carry on has viable bacteria count, no matter be the bacterium fluid that fosters 14h or 24h,be ultrasonic fragmentation test ends inside 6h, the nature that takes no account of a bacterium dies.
The fluid of bacterium of bacili of gemma of Su Yun gold of 2.1 education 14h is broken the lever of broken result luffing that expresses fluid of bacterium of 1 education 14h as a result average viable bacteria leads working hours of chroma of 5 bacterium fluid average and broken rate (％ ) * as working hours increase, naked eye observes turbidity of visible bacterium fluid is reduced and diaphaneity increases.
Different bacterial chroma, with same luffing lever (56 or 510) , inside same working hours, 5 billion / the broken rate of Ml is compared 10 billion / the tall 1.75~8 of Ml percent; Identical bacterial chroma (5 billion / Ml or 10 billion / Ml) , with different luffing lever (56 and 510) achieve same broken rate time to be in basically 10min left and right sides, but no matter use 56 or the luffing lever of 510, or 5 billion / Ml or 10 billion / the bacterial chroma of Ml, achieve 95% above broken rate is needed to be with time inside 50min.
Broken result spends the fluid of bacterium of bacili of gemma of Su Yun gold of 2.2 education 24h, also no matter use 56 or the luffing lever of 510, inside the working hours of 30~60min, the broken rate of bacterium fluid is in 6. Between 5~12% , if consider the little share inside fluid of bacterium of bacili of gemma of gold of education 24h Su Yun (about 10% the left and right sides) outside nutrient cell and computational error, can think ultrasonic is right completely of gemma of bacili of gemma of gold of education 24h Su Yun broken without apparent effect.
The lever of broken result luffing that expresses fluid of bacterium of 2 education 24h average viable bacteria leads working hours of chroma of 5 bacterium fluid average and broken rate (％ ) the * addition as working hours, naked eye observes turbidity of visible bacterium fluid does not have apparent change.
As the development of biology project technology, the application of bacili of gemma of Su Yun gold is becoming wider and wider, and find its effective broken method, have very important theory and practice sense. We are commonly used in working with research in the experiment ultrasonic comes broken cell in order to depart cellular composition or use at immune chemistry to consider. This second experiment makes clear, although whether forming gemma is the genetic character that bacterium plants, but if develop condition of time, nutrition,also wait with ambient conditions at the same time relative. The bacterium of bacili of gemma of Su Yun gold that has not produced gemma to fostering 14h for fluid, ultrasonic is broken the law falls in experiment condition, no matter choose *6 luffing lever or diameter relative to larger 510 luffing staff, also no matter bacterium body chroma is 5 billion / Ml, or 10 billion / Ml, what the broken rate that achieves 95% above needs time to be 30~ 50min, broken effect is relatively ideal; The juice of bacterium of bacili of gemma of Su Yun gold that has produced gemma to fostering 24h however will tell, there is gemma already in bacterium fluid, still have partial nutrition cell, from the watch 2 can see, broken rate all inferior, ultrasonic is broken the effect of the law is extremely indistinctive.
Ultrasonic is the sound wave that oscillatory frequency exceeds 20 thousand Hz.
It can make through intense vibration cellular burst, the discharge outside cellular inclusion and die. The element such as the broken effect of ultrasonic and processing power, frequency, frequency, time, microbial type and its physiology condition is concerned. Of gemma fight go against a gender strong, suffer the effect that ultrasonic handles scarcely, the broken appropriate of reason gemma uses other method. Check makes clear Bencishi: Applied ultrasonic handles bacili of gemma of Su Yun gold to answer special attention condition of time of this bacterium education, education and biology condition.
This second experiment is used blame destroy vivid cell is broken, relatively strict to environmental requirement, but carry bacterial count, can master each time explicitly quite paragraph cell (include dormancy cell - gemma) broken case, as to destroy body of live bacterium of bacili of gemma of Su Yun gold reachs its gemma first-rate broken method and broken case, still remain to study further.
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